H2N2V1, Main, Exploration, bibRecord, 001003

Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.

Identifieur interne : 001003 ( Main/Exploration ); précédent : 001002; suivant : 001004

Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.

Auteurs : Peter P. Cheung [République populaire de Chine] ; Y H Connie Leung [République populaire de Chine] ; Chun-Kin Chow [République populaire de Chine] ; Chi-Fung Ng [République populaire de Chine] ; Chun-Lok Tsang [République populaire de Chine] ; Yu-On Wu [République populaire de Chine] ; Siu-Kit Ma [République populaire de Chine] ; Sin-Fun Sia [République populaire de Chine] ; Yi Guan [République populaire de Chine] ; J. S. M. Peiris [République populaire de Chine]

Source :

Journal of Clinical Virology [ 1386-6532 ] ; 2009-09.

RBID : Hal:pasteur-00588920

Abstract

BACKGROUND: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. OBJECTIVES: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. STUDY DESIGN: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. RESULTS: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8mug of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. CONCLUSION: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds.

Url:

https://hal-riip.archives-ouvertes.fr/pasteur-00588920

DOI: 10.1016/j.jcv.2009.06.016

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000143
to stream Hal, to step Curation: 000143
to stream Hal, to step Checkpoint: 000287
to stream Main, to step Merge: 001011
to stream Main, to step Curation: 001003

Le document en format XML

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            <ref type="url">http://www.hku.hk/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-136023" status="VALID">          <orgName>Department of Microbiology [HKU]</orgName>
          <desc>            <address>              <addrLine>Hong Kong Special Administrative Region, China</addrLine>
              <country key="CN"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-93487" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-93487" type="direct"><org type="institution" xml:id="struct-93487" status="VALID">          <orgName>The University of Hong Kong</orgName>
          <orgName type="acronym">HKU</orgName>
          <desc>            <address>              <addrLine>Pokfulam, Hong Kong</addrLine>
              <country key="HK"></country>
            </address>
            <ref type="url">http://www.hku.hk/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-136023" status="VALID">          <orgName>Department of Microbiology [HKU]</orgName>
          <desc>            <address>              <addrLine>Hong Kong Special Administrative Region, China</addrLine>
              <country key="CN"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-93487" type="direct"></relation>
          </listRelation>
        <tutelles><tutelle active="#struct-93487" type="direct"><org type="institution" xml:id="struct-93487" status="VALID">          <orgName>The University of Hong Kong</orgName>
          <orgName type="acronym">HKU</orgName>
          <desc>            <address>              <addrLine>Pokfulam, Hong Kong</addrLine>
              <country key="HK"></country>
            </address>
            <ref type="url">http://www.hku.hk/</ref>
          </desc>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1016/j.jcv.2009.06.016</idno>
<series><title level="j">Journal of Clinical Virology</title>
<idno type="ISSN">1386-6532</idno>
<imprint><date type="datePub">2009-09</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>BACKGROUND: Avian influenza virus (AIV) surveillance in birds is important for public health. Faecal droppings from wild-birds are more readily available for such studies, but the inability to identify the species-origin of faecal samples limits their value. OBJECTIVES: To develop, optimise, and field-test a method to simultaneously detect AIV and identify the species-origin from faecal samples. STUDY DESIGN: Analytical sensitivity of the species-identification RT-PCR was assessed on serial dilutions of faecal droppings. Overall sensitivity of the methods for species-identification and AIV detection was assessed on 92 faecal and cloacal samples collected from wildlife, poultry markets, and experimentally H5N1-infected birds. RESULTS: All 92 samples were correctly identified to 24 different species, with a detection limit of 2.8mug of faecal material. All 20 specimens previously shown by virus culture to be positive for influenza virus were correctly identified by RT-PCR for influenza A using the same nucleic-acid extracts used for species-identification. CONCLUSION: We have optimised and evaluated a method for identifying the species of origin and detecting AIV from bird faecal droppings that can be applied to routine surveillance of influenza viruses in wild-birds.</p>
            </div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Cheung, Peter P" sort="Cheung, Peter P" uniqKey="Cheung P" first="Peter P" last="Cheung">Peter P. Cheung</name>
</noRegion>
<name sortKey="Chow, Chun Kin" sort="Chow, Chun Kin" uniqKey="Chow C" first="Chun-Kin" last="Chow">Chun-Kin Chow</name>
<name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name>
<name sortKey="Leung, Y H Connie" sort="Leung, Y H Connie" uniqKey="Leung Y" first="Y H Connie" last="Leung">Y H Connie Leung</name>
<name sortKey="Ma, Siu Kit" sort="Ma, Siu Kit" uniqKey="Ma S" first="Siu-Kit" last="Ma">Siu-Kit Ma</name>
<name sortKey="Ng, Chi Fung" sort="Ng, Chi Fung" uniqKey="Ng C" first="Chi-Fung" last="Ng">Chi-Fung Ng</name>
<name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
<name sortKey="Sia, Sin Fun" sort="Sia, Sin Fun" uniqKey="Sia S" first="Sin-Fun" last="Sia">Sin-Fun Sia</name>
<name sortKey="Tsang, Chun Lok" sort="Tsang, Chun Lok" uniqKey="Tsang C" first="Chun-Lok" last="Tsang">Chun-Lok Tsang</name>
<name sortKey="Wu, Yu On" sort="Wu, Yu On" uniqKey="Wu Y" first="Yu-On" last="Wu">Yu-On Wu</name>
</country>
</tree>
</affiliations>
</record>

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Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.

Identifying the species-origin of faecal droppings used for avian influenza virus surveillance in wild-birds.

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